Human lambda light chain mice,申请号US201414561867-传众专利搜索

Human lambda light chain mice

申请公布号：US9394373(B2)

申请号：US201414561867

申请日期：2014.12.05

申请公布日期：2016.07.19

申请人：

Regeneron Pharmaceuticals, Inc.

发明人：Macdonald Lynn;Stevens Sean;Gurer Cagan;Murphy Andrew J.;Meagher Karolina A.

分类号：C12N15/01;C07K16/46;A01K67/027;C07K16/00;C12N15/85;C07K16/18;C12N15/10

主分类号：C12N15/01

代理机构：

Foley Hoag LLP

代理人：Jones Brendan T.;Miyamoto Neil;Foley Hoag LLP

地址：Tarrytown NY US

摘要：Genetically modified mice are provided that express human λ variable (hVλ) sequences, including mice that express hVλ sequences from an endogenous mouse λ light chain locus, mice that express hVλ sequences from an endogenous mouse κ light chain locus, and mice that express hVλ sequences from a transgene or an episome wherein the hVλ sequence is linked to a mouse constant sequence. Mice are provided that are a source of somatically mutated human λ variable sequences useful for making antigen-binding proteins. Compositions and methods for making antigen-binding proteins that comprise human λ variable sequences, including human antibodies, are provided.

主权项：1. A transgenic mouse comprising in its genome an endogenous λ light chain immunoglobulin locus comprising a deletion of the endogenous mouse Vλ1-Jλ3-Cλ3-Jλ1-Cλ1 gene cluster and a deletion of a region spanning from Vλ2 to Jλ2 of the endogenous mouse Vλ2-Vλ3-Jλ2-Cλ2-Jλ4-Cλ4 gene cluster.