Modified human tumor necrosis factor receptor-1 polypeptide or fragment thereof, and method for preparing same,申请号US201113513803-传众专利搜索

Modified human tumor necrosis factor receptor-1 polypeptide or fragment thereof, and method for preparing same

申请公布号：US8754037(B2)

申请号：US201113513803

申请日期：2011.12.21

申请公布日期：2014.06.17

申请人：

Hanall Biopharma Co., Ltd.

发明人：Kim Sung Wuk;Jun Sung Soo;Park Seung Kook;Kim Song Young;Kim Eun Sun;Jeong Jae Kap;Kim Ha Na;Song Yeon Jung

分类号：C07K14/715;C12N15/70;A61K38/17;A61P5/00;A61P11/00;A61P3/04;A61P3/10;A61P19/02;A61P19/08;A61P25/08;A61P35/00;A61P27/02;A61K31/7088;C07H21/04;A61P41/00;A61P29/00;A61P1/00;A61P25/28;A61P1/18;C12N1/21

主分类号：C07K14/715

代理机构：

Sughrue Mion, PLLC

代理人：Sughrue Mion, PLLC

地址：Daejeon KR

摘要：Provided is a modified human tumor necrosis factor receptor-1 polypeptide or a fragment thereof that binds to a tumor necrosis factor in vivo or ex vivo. The modified human tumor necrosis factor receptor-1 polypeptide or fragment exhibits improved ability to bind tumor necrosis factor and resistance to proteases.

主权项：1. A modified tumor necrosis factor receptor-1 polypeptide, comprising an amino acid sequence selected from the group consisting of: an amino acid sequence comprising modifications of 5 amino acid residues consisting of 4 amino acid residues at positions 92, 95, 97 and 98, and 1 amino acid residue at one selected from among positions 68, 161, 200 and 207 in an amino acid sequence of a wild-type tumor necrosis factor receptor-1 polypeptide represented by SEQ ID NO: 1; an amino acid sequence comprising modifications of 5 amino acid residues consisting of 4 amino acid residues at positions 92, 95, 97 and 98, and 1 amino acid residue at one selected from among positions 68, 161, 200 and 207 in an amino acid sequence (TNFRI171) consisting of amino acids 41-211 of an amino acid sequence of a wild-type tumor necrosis factor receptor-1 polypeptide represented by SEQ ID NO: 1; an amino acid sequence comprising modifications of 5 amino acid residues consisting of 4 amino acid residues at positions 92, 95, 97 and 98, and 1 amino acid residue at one selected from among positions 68 and 161 in an amino acid sequence (TNFRI126) consisting of amino acids 41-166 of an amino acid sequence of a wild-type tumor necrosis factor receptor-1 polypeptide represented by SEQ ID NO: 1; and an amino acid sequence comprising modifications of 5 amino acid residues at positions 68, 92, 95, 97 and 98 in an amino acid sequence (TNFRI105) consisting of amino acids 41-145 of an amino acid sequence of a wild-type tumor necrosis factor receptor-1 polypeptide represented by SEQ ID NO: 1.