Methods, compositions and kits for the improved detection of small RNA molecules

申请公布号：US8637276(B2)

申请号：US20080682344

申请日期：2008.10.08

申请公布日期：2014.01.28

申请人：

KUTYAVIN IGOR VASSILY

发明人：KUTYAVIN IGOR VASSILY

分类号：C12P19/34;B01L3/00;C07G3/00;C07H21/04;C12Q1/68

主分类号：C12P19/34

摘要：The present invention provides compositions, methods and kits for use in the detection of small RNA sequences, which allow for rapid and robust amplification and detection. The methods provide improved sensitivity and efficiency in the amplification-based detection of small RNA sequences by incorporating one or more base-modified duplex-stabilizing dNTPs during reverse transcription and/or amplification.

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