BACTERIA WITH REDUCED GENOME,申请号CA20032473922-传众专利搜索

BACTERIA WITH REDUCED GENOME

申请公布号：CA2473922(C)

申请号：CA20032473922

申请日期：2003.01.22

申请公布日期：2013.09.24

申请人：

WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人：BLATTNER, FREDERICK R.;POSFAI, GYORGY;HERRING, CHRISTOPHER D.;PLUNKETT, GUY D., III;GLASNER, JEREMY D.;TREVOR, TWOSE

分类号：C12N1/21;C12N15/09;C12N1/08;C12N1/20;C12N15/10;C12N15/70;C12N15/74;C12N15/90;C12P21/02;C12R1/19

主分类号：C12N1/21

摘要：The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to about 20% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.