METHOD TO SCREEN HIGH AFFINITY ANTIBODY
申请公布号:KR101252362(B1)
申请号:KR20117004500
申请日期:2009.08.25
申请公布日期:2013.04.08
分类号:G01N21/55;G01N33/52;G01N33/53;G06F19/10
主分类号:G01N21/55
摘要:The current invention reports a method for producing an antibody comprising the steps of a) providing a plurality of hybridoma cells each expressing an antibody, b) determining the time dependent amount of said antibody bound to the respective antigen by surface plasmon resonance at different temperatures and different antibody concentrations, c) calculating with the time dependent amount determined in b) based on equations (II) to (XIII) at least the thermodynamic parameters (i) standard association binding entropy (”S°€¡ass), (ii) standard dissociation binding entropy (”S°€¡diss), (iii) standard binding entropy (”S°), (iv) free standard binding enthalpy (”G°), (v) standard dissociation free binding enthalpy (”G°€¡diss), (vi) standard association free binding enthalpy (”S°€¡ass), (vii) -T”S°, (viii) dissociation rate constant k d , (ix) equilibrium binding constant K D , and (x) association rate constant k a , d) selecting a hybridoma cell producing an antibody with at least two of the following: i) a standard association binding entropy of less than 10 J/K*mol, ii) an absolute standard dissociation binding entropy of 100 J/mol*K or more, iii) an absolute standard binding entropy of 100 J/mol*K or more, e) producing an antibody by cultivating said selected cell under conditions suitable for the expression of said antibody and recovering said antibody from the cells or/and the cultivation medium
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