Simultaneous detection of metabolic enzyme activity and metabolite levels,申请号US20090349669-传众专利搜索

Simultaneous detection of metabolic enzyme activity and metabolite levels

申请公布号：US8318417(B2)

申请号：US20090349669

申请日期：2009.01.07

申请公布日期：2012.11.27

申请人：

CERDA BLAS;PERKINELMER HEALTH SCIENCES, INC.

发明人：CERDA BLAS

分类号：C12Q1/00;C12Q1/34;C12Q1/68;G01N33/00;G01N33/53

主分类号：C12Q1/00

摘要：Provided are methods for detecting a metabolic disorder in an individual using mass spectrometry. One method involves (a) contacting a sample containing (i) a metabolically indicative enzyme and (ii) a metabolic analyte, with a substrate for the enzyme to produce a reaction admixture, under conditions wherein the enzyme is capable of acting on a corresponding substrate to generate a product, and wherein a protease inhibitor is present; (b) contacting the reaction admixture with a reagent that inhibits the ability of the enzyme to act on a corresponding substrate, wherein the metabolic analyte and the product are soluble in the reagent; to produce a test sample and (c) determining the presence or amount of the metabolic analyte and the product contained in the test sample using mass spectrometry, wherein a determined presence or amount of the metabolic analyte and the product correlates with presence or absence of the metabolic disorder.