Method for melting analysis of nucleic acids,申请号EP20080731668-传众专利搜索

Method for melting analysis of nucleic acids

申请公布号：EP2134861(B1)

申请号：EP20080731668

申请日期：2008.03.07

申请公布日期：2012.04.18

申请人：

IDAHO TECHNOLOGY;UNIVERSITY OF UTAH RESEARCH FOUNDATION

发明人：WITTWER, CARL, T.;ZHOU, LUMING;PORITZ, MARK, AARON

分类号：C12Q1/68

主分类号：C12Q1/68

摘要：Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5′ of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.