Oligonucleotide pairs for multiplexed binding assays

申请公布号：US7195875(B2)

申请号：US20030419020

申请日期：2003.04.18

申请公布日期：2007.03.27

申请人：

BECKMAN COULTER, INC.

发明人：KEYS DANIEL A.;REDDY PARAMESWARA MEDA;BODNAR JACKIE S.;FAROOQUI FIRDOUS

分类号：C12Q1/68;C07H21/04;C12M1/34;G01N33/543

主分类号：C12Q1/68

摘要：The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.

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