Production of functional proteins: balance of shear stress and gravity,申请号US20030734759-传众专利搜索

Production of functional proteins: balance of shear stress and gravity

申请公布号：US2005064443(A1)

申请号：US20030734759

申请日期：2003.12.11

申请公布日期：2005.03.24

申请人：

USA AS REPRESENTED BY THE ADMINISTRATOR, NATIONAL AERONAUTICS & SPACE ADMINISTRATION

发明人：GOODWIN THOMAS JOHN;HAMMOND TIMOTHY GRANT;KAYSEN JAMES HOWARD

分类号：A61K38/00;C12N5/00;C12N15/113;C12N15/67;(IPC1-7):C12Q1/68

主分类号：A61K38/00

摘要：The present invention provides a method for production of functional proteins including hormones by renal cells in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel. Natural mixture of renal cells expresses the enzyme 1-a-hydroxylase which can be used to generate the active form of vitamin D: 1,25-diOH vitamin D3. The fibroblast cultures and co-culture of renal cortical cells express the gene for erythropoietin and secrete erythropoietin into the culture supernatant. Other shear stress response genes are also modulated by shear stress, such as toxin receptors megalin and cubulin (gp280). Also provided is a method of treating in-need individual with the functional proteins produced in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel.