High-throughput DNA methylation profiling and comparative analysis,申请号US20040835028-传众专利搜索

High-throughput DNA methylation profiling and comparative analysis

申请公布号：US2004203048(A1)

申请号：US20040835028

申请日期：2004.04.28

申请公布日期：2004.10.14

申请人：

TRAN NATHANIEL TUE

发明人：TRAN NATHANIEL TUE

分类号：C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68

主分类号：C12P19/34

摘要：This invention teaches high-throughput process for quantifying and comparing DNA methylation in multiple genes. DNA is extracted and subject to treatments that differentially modify either non-methylated nucleotides or methylated nucleotides. The modification results in labels or detectable tags that can easily be detected and quantified. The treated DNA is then digested by restriction enzymes and profiled on DNA array. The method can be used to compare two samples of DNA to look for differentially methylated genes. The method can also reveal polymorphism besides epigenetic differences.