Culture medium for avian totipotential embryonic cells,申请号EP20010108584-传众专利搜索

Culture medium for avian totipotential embryonic cells

申请公布号：EP1149899(A1)

申请号：EP20010108584

申请日期：1995.10.20

申请公布日期：2001.10.31

申请人：

INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS);ECOLE NORMALE SUPERIEURE DE LYON

发明人：SAMARUT, JACQUES;PAIN, BERTRAND

分类号：C12N5/0735;A01K67/027;C12N5/00;C12N5/02;C12N5/073;(IPC1-7):C12N5/06;C12N5/10

主分类号：C12N5/0735

摘要：<p>Culture medium for totipotent, embryonic avian cells (embryonic stem cells, ESC) comprises a medium for avian cell culture supplemented with cytokines, fibroblast growth factor (FGF), insulin-like growth factor (IGF) and stem cell growth factor (SC). The medium is practically free of active retinoic acid. Also claimed are:(1) in vitro cultures of avian ESC produced using this medium;(2) ESC modified by introducing a gene that encodes a heterologous protein into the genome of an avian ESC in culture, and(3) transgenic animal derived at least partly from such cells.</p>