Stimulation of angiogenesis via enhanced endothelial expression of syndecan-4 core proteins,申请号AU19990056794-传众专利搜索

Stimulation of angiogenesis via enhanced endothelial expression of syndecan-4 core proteins

申请公布号：AU5679499(A)

申请号：AU19990056794

申请日期：1999.08.18

申请公布日期：2000.03.27

申请人：

BETH ISRAEL DEACONESS MEDICAL CENTER

发明人：MICHAEL SIMONS;RUDIGER VOLK;ARIE HOROWITZ

分类号：A61K48/00;C07K14/47;C07K14/705;C12N15/08

主分类号：A61K48/00

摘要：The present invention provides tangible means and methods for stimulation of angiogenesis via enhanced endothelial expression of core proteins having a syndecan-4 cytoplasmic region intracellularly. The tangible means include a prepared DNA sequence fragment having separate and individual DNA sequenced portions coding for an heparan sulfate binding extracellular domain, a central transmembrane domain, and a cytoplasmic domain coding for the syndecan-4 polypeptide. The prepared DNA sequence unitary fragment may be delivered to endothelial cells in-situ, both under in-vivo and/or in-vitro conditions, using suitable expression vectors including plasmids and viruses. The resulting transfected endothelial cells overexpress heparan sulfate binding, core proteins; and the resulting overexpression of these proteoglycan entities causes stimulation of angiogenesis in-situ.