Oil bodies and associated proteins as affinity matrices
申请公布号:AU5220498(A)
申请号:AU19980052204
申请日期:1997.12.05
申请公布日期:1998.07.15
发明人:MAURICE MOLONEY;JOSEPH BOOTHE;GIJS VAN ROOIJEN
分类号:B01D15/08;C01G11/00;C07H21/00;C07H21/04;C07K1/22;C07K14/415;C07K16/06;C07K17/02;C07K19/00;C08B1/00;C12N9/74;C12S3/14
主分类号:B01D15/08
摘要:A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
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