DRY-TYPED MULTILAYER ANALYSIS ELEMENT TO ASSAY TRANSAMINASE
申请公布号:JPH0819398(A)
申请号:JP19950000027
申请日期:1995.01.04
申请公布日期:1996.01.23
发明人:MAASHIYA DENISU BEERU OONITSUKU;RICHIYAADO RIN DETSUTOWAIRAA;JIYON NASAN EIKENBERII;JIYON UIRIAMU HENDAASON SAZAARANDO
分类号:G01N31/22;C12Q1/00;C12Q1/26;C12Q1/52;G01N21/78;G01N33/50;G01N33/52;(IPC1-7):C12Q1/52
主分类号:G01N31/22
摘要:A dry multilayer analytical element for assaying transaminases having, in the following order, a spreading layer, a reagent layer and a support and the element comprises: a) a transaminase substrate; b) reagents that generate pyruvate in the presence of a transaminase and the transaminase substrate; c) pyruvate oxidase and cofactors for pyruvate oxidase; d) reagents that generate a color in proportion to a concentration of hydrogen peroxide; characterized in that: e) the spreading layer contains the transaminase substrate and a material selected from the group consisting of polymer beads, barium sulfate, titanium dioxide and mixtures thereof; f) the reagent layer contains the pyruvate oxidase; and g) at least one layer separate the spreading layer from the reagent layer.
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