PROCESS FOR THE PURIFICATION OF BIOLOGICALLY ACTIVE PROTEINS
申请公布号:RO97846(B1)
申请号:RO19860125165
申请日期:1986.10.27
申请公布日期:1990.01.30
发明人:LEO SHUN;RALPH YAMAMOTO;GLENN DORIN;WOLFGANG HELMUT HANISCH
分类号:C07K1/00
主分类号:C07K1/00
摘要:<p>Prezenta inventie se refera la un procedeu de purificare a proteinelor biologic active de tip TNF matur recombinant, TNF matur recombinant modificat prin deletii N-terminale, conform caruia celulele care au sintetizat TNF se recolteaza, se sfarâma prin sonicare, se aplica pe o coloana de cromatografie cu schimbatori de anioni, de tipul DEAE agaroza, fractiunile cu activitate de TNF se colecteaza si se aplica pe o matrice cromatografica de interactiune hidrofoba, mentinuta la aceeasi temperatura ca si amestecul tampon continând TNF, din care, dupa elutie cu un gradient liniar, se obtin fractiuni continând TNF, care, în final, se purifica.</p>
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