UN PROCEDIMIENTO PARA LA PURIFICACION DE QUIMOPAPAINA BRUTA.,申请号ES19130005121-传众专利搜索

UN PROCEDIMIENTO PARA LA PURIFICACION DE QUIMOPAPAINA BRUTA.

申请公布号：ES512113(D0)

申请号：ES19130005121

申请日期：1982.05.12

申请公布日期：1983.02.01

申请人：

SMITH LABORATORIES, INC.

分类号：A61K38/00;C12N9/50;(IPC1-7):07G7/00;61K31/54

主分类号：A61K38/00

摘要：<p>A method of preparing a purified proteolytically active chymopapain from crude chymopapain extracts by the use of exchange resin absorption purification techniques is provided. The purified chymopapain is characterized by relative freedom from proteolytically inactive, colored and/or toxic components and by its failure to form a precipitate with barium chloride test solution (U.S.P.) under acid conditions. An improved method for treating an intervertebral spinal disc in a mammalian subject which disc exhibits an abnormal condition, characterized by a reduced risk of toxic effect to the subject is also provided which comprises, injecting in said disc an amount of a pharmaceutically acceptable solution of a purified chymopapain essentially free of proteolytically inactive and/or toxic protein components, said purified chymopapain being used in an amount sufficient to selectively dissolve the nucleus pulposus of said disc. Pharmaceutical compositions are also provided for use in said method which include a purified proteolytically active lyophilized chymopapain, essentially free of proteolytically inactive and toxic components, and an activating amount of a pharmaceutically acceptable reducing agent.</p>