NOVEL SELECTION VECTORS AND METHODS OF SELECTING EUKARYOTIC HOST CELLS

申请公布号：EP3027646(A1)

申请号：EP20140753158

申请日期：2014.07.29

申请公布日期：2016.06.08

申请人：

NOVARTIS AG

发明人：ASSARAF, YEHUDA G.;JOSTOCK, THOMAS;KNOPF, HANS-PETER

分类号：C07K14/705;C12N15/65;C12N15/69;C12N15/85

主分类号：C07K14/705

摘要：The disclosure pertains to an expression vector or a combination of at least two expression vectors comprising: a) a polynucleotide encoding a mutated folate receptor as selectable marker, wherein the mutated folate receptor has a decreased folate binding affinity compared to the wild type folate receptor; b) at least one polynucleotide encoding a polypeptide of interest, wherein when said expression vector or combination of at least two expression vectors is introduced into a host cell, the polypeptide of interest is secreted from said host cell. Also provided are suitable host cells, selection methods and methods for producing polypeptides with high yield.

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