Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping,申请号US201012690579-传众专利搜索

Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping

申请公布号：US9285575(B2)

申请号：US201012690579

申请日期：2010.01.20

申请公布日期：2016.03.15

申请人：

PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人：Xie Xiaoliang Sunney;Freudiger Christian;Min Wei

分类号：G02B21/00;G01J3/10;G01J3/44;G01N21/65;G02B21/08;G01J3/12

主分类号：G02B21/00

代理机构：

Gesmer Updegrove LLP

代理人：Gesmer Updegrove LLP

地址：Cambridge MA US

摘要：A microscopy imaging system is disclosed that includes a light source system, a spectral shaper, a modulator system, an optics system, an optical detector and a processor. The light source system is for providing a first train of pulses and a second train of pulses. The spectral shaper is for spectrally modifying an optical property of at least some frequency components of the broadband range of frequency components such that the broadband range of frequency components is shaped producing a shaped first train of pulses to specifically probe a spectral feature of interest from a sample, and to reduce information from features that are not of interest from the sample. The modulator system is for modulating a property of at least one of the shaped first train of pulses and the second train of pulses at a modulation frequency. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of a train of pulses of interest transmitted or reflected through the common focal volume. The processor is for detecting a modulation at the modulation frequency of the integrated intensity of substantially all of the optical frequency components of the train of pulses of interest due to the non-linear interaction of the shaped first train of pulses with the second train of pulses as modulated in the common focal volume, and for providing an output signal for a pixel of an image for the microscopy imaging system.

主权项：1. A microscopy imaging system comprising: a light source system for providing a first train of pulses including a first broadband range of frequency components, and a second train of pulses including a second optical frequency such that a set of differences between the first broadband range of frequency components and the second optical frequency is resonant with a set of vibrational frequencies of a sample in the focal volume, wherein the second train of pulses is synchronized with the first train of pulses; a spectral shaper including a dispersing element for spectrally dispersing frequency components of the broadband range of frequency components, spectrally modifying an optical property of at least some frequency components of the broadband range of frequency components, and spectrally combining using the dispersing element, the modified frequency components such that the broadband range of frequency components is shaped producing a shaped first train of pulses to specifically probe a spectral feature of interest from a sample, and to reduce information from features that are not of interest from the sample; a modulator system for modulating a property of at least one of the shaped first train of pulses and the second train of pulses at a modulation frequency to provide a modulated train of pulses; an optics system for directing and focusing the shaped first train of pulses and the second train of pulses as modulated toward a common focal volume; an optical detector for detecting an integrated intensity of substantially all optical frequency components of a train of pulses of interest transmitted or reflected through the common focal volume; and a processor for detecting a modulation at the modulation frequency of the integrated intensity of substantially all of the optical frequency components of the train of pulses of interest due to the non-linear interaction of the shaped first train of pulses with the second train of pulses as modulated in the common focal volume, and for providing an output signal for a pixel of an image for the microscopy imaging system; wherein only one of the shaped train of laser pulses or the second train of pulses is modulated at the modulation frequency to provide the modulated train of pulses such that the other of the shaped train of laser pulses and the second train of pulses remains a non-modulated train of pulses; wherein the optical detector detects the integrated intensity of substantially all optical frequency components of the non-modulated train of pulses transmitted or reflected through the common focal volume by blocking the modulated train of pulses; and wherein the processor detects a modulation at the modulation frequency of the integrated intensity of substantially all of the optical frequency components of the non-modulated train of pulses due to the non-linear interaction of the modulated train of pulses with the non-modulated train of pulses in the common focal volume.