Method to Perform Beam-Type Collision-Activated Dissociation in the Pre-Existing Ion Injection Pathway of a Mass Spectrometer,申请号US201514703643-传众专利搜索

Method to Perform Beam-Type Collision-Activated Dissociation in the Pre-Existing Ion Injection Pathway of a Mass Spectrometer

申请公布号：US2016020081(A1)

申请号：US201514703643

申请日期：2015.05.04

申请公布日期：2016.01.21

申请人：

Wisconsin Alumni Research Foundation

发明人：COON Joshua J.;MCALISTER Graeme C.

分类号：H01J49/10;H01J49/00

主分类号：H01J49/10

地址：Madison WI US

摘要：Described herein are methods and systems related to the use of the pre-existing ion injection pathway of a mass spectrometer to perform beam-type collision-activated dissociation, as well as other dissociation methods. The methods can be practiced using a wide range of mass spectrometer configurations and allows MSn experiments to be performed on very basic mass spectrometers, even those without secondary mass analyzers and/or collision cells. Following injection and selection of a particular ion type or population, that population can be fragmented via beam-type collision-activated dissociation (CAD), as well as other dissociation methods, using the pre-existing ion injection pathway or inlet of a mass spectrometer. For CAD applications, this is achieved by transmitting the ions back along the ion injection pathway with a high degree of kinetic energy. As the ions pass into the higher pressure regions located in or near the atmospheric pressure inlet, the ions are fragmented and then trapped. Following fragmentation and trapping, the ions can either be re-injected into the primary ion selection device or sent on to a secondary mass analyzer.

主权项：1. A method for generating product ions, the method comprising: providing ions from an ion source through an inlet into an ion injection pathway having ion injection pathway ion optics; transmitting the ions along a first direction away from the inlet through the ion injection pathway into an ion selection device having ion selection device ion optics, wherein the ion injection pathway ion optics comprise two or more multipole RF devices and one or more ion lens devices, wherein the multipole RF devices and the ion lens devices are provided between the inlet and the ion selection device and wherein at least one ion lens device is provided between adjacent multipole RF devices; selecting a subset of the ions in the ion selection device; wherein the subset of the ions have a preselected range of mass-to-charge ratios; and transmitting the subset of the ions having the preselected range of mass-to-charge ratios along a second direction toward the inlet through the ion injection pathway; re-injecting a portion of the subset of the ions transmitted along the second direction into the inlet or into one of the two or more multipole RF devices provided between the inlet and the ion selection device, wherein the transmitting steps and selecting steps are repeated one or more times; wherein the ion injection pathway ion optics have an RF voltage component and a DC voltage component which are under independent control with respect to the ion selection device ion optics; and wherein at least a portion of the subset of the ions transmitted along the second direction interact with one or more gases at a pressure greater than 0.01 Torr in the inlet or ion injection pathway and undergo dissociation, thereby fragmenting at least a portion of the subset of the ions having the preselected range of mass-to-charge ratios to generate the product ions.