DETECTION SOLUTION, METHOD FOR PREPARING SAME, AND BIOSENSING METHOD,申请号WO2015JP02062-传众专利搜索

DETECTION SOLUTION, METHOD FOR PREPARING SAME, AND BIOSENSING METHOD

申请公布号：WO2015159535(A1)

申请号：WO2015JP02062

申请日期：2015.04.14

申请公布日期：2015.10.22

申请人：

FUJIFILM CORPORATION

发明人：OHTSUKA, HISASHI;SAIKI, TOSHIHARU;ESASHIKA, KEIKO

分类号：C12Q1/68;C12N15/09;G01N21/21;G01N21/47;G01N27/447;G01N33/53;G01N33/543

主分类号：C12Q1/68

摘要：[Problem] To improve detection accuracy in biosensing using precious metal nanoparticles as a marker. [Solution] This detection solution (1) is made by dispersing first nanoparticle probes (P1) and second nanoparticle probes (P2) in a buffer aqueous solution (40). Each first nanoparticle probe (P1) is made by fixing, to the surface of a first precious metal nanoparticle (10A), a first probe (20A) including a biological substance that specifically binds to a first section (50A) of a substance to be detected (50). Each second nanoparticle probe (P2) is made by fixing, to the surface of a second precious metal nanoparticle (10B), a second probe (20B) including a biological substance that specifically binds to a second section (50B) of the substance to be detected (50). The first probe (20A) and the second probe (20B) are fixed to the first precious metal nanoparticle (10A) and the second precious metal nanoparticle (10B), respectively, such that the respective base ends (20Ab (20Bb)) of the first probe (20A) and the second probe (20B) specifically bind, respectively, to the respective leading ends (50At (50Bt)) of the first section (50A) and the second section (50B) on the side where the two section are proximal to one another.