Methods for identifying small molecules that bind specific RNA structural motifs,申请号US20060359721-传众专利搜索

Methods for identifying small molecules that bind specific RNA structural motifs

申请公布号：US2006194234(A1)

申请号：US20060359721

申请日期：2006.02.21

申请公布日期：2006.08.31

申请人：

PTC THERAPEUTICS, INC.

发明人：CONN MICHAEL M.;PELLIGRINI MATHEW;HWANG SEONGWOO;MOON YOUNG-CHOON;ALMSTEAD NEIL

分类号：C40B40/08;C12N15/10;C12Q1/68

主分类号：C40B40/08

摘要：The present invention relates to a method for screening and identifying test compounds that bind to a preselected target ribonucleic acid ("RNA"). Direct, non-competitive binding assays are advantageously used to screen bead-based libraries of compounds for those that selectively bind to a preselected target RNA. Binding of target RNA molecules to a particular test compound is detected using any physical method that measures the altered physical property of the target RNA bound to a test compound. The structure of the test compound attached to the labeled RNA is also determined. The methods used will depend, in part, on the nature of the library screened. The methods of the present invention provide a simple, sensitive assay for high-throughput screening of libraries of compounds to identify pharmaceutical leads.