Hyaluronic Acid Derivative, Method of Preparation Thereof, Method of Modification Thereof and Use Thereof,申请号US201314420012-传众专利搜索

Hyaluronic Acid Derivative, Method of Preparation Thereof, Method of Modification Thereof and Use Thereof

申请公布号：US2015175717(A1)

申请号：US201314420012

申请日期：2013.08.08

申请公布日期：2015.06.25

申请人：

Contipro Biotech s.r.o.

发明人：Buffa Radovan;Sedova Petra;Velebny Vladimir;Wolfova Lucie;Basarabova Ivana;Pospisil Robert;Hasova Martina;Nesporova Kristina

分类号：C08B37/08;A61K47/48;C07K17/10

主分类号：C08B37/08

地址：Dolni Dobrouc CZ

摘要：The invention relates to the preparation and use of α,β-unsaturated aldehyde of hyaluronan having a double bond in the positions 4 and 5 and an aldehydic group in the position 6 of the glucosamine part of the polysaccharide. The method of preparation is based on dehydration of hyaluronan having an aldehydic group in the position 6 of the glucosamine part of the polysaccharide. Two methods have been described, which are dehydration in a solution or heating in solid state in absence of solvents, bases or other additives. This derivative allows stabilization of conjugates of hyaluronan with amino compounds by means of a multiple bond from the aldehyde side, and therefore, it is possible to effectively immobilize practically any compound containing an amino group to such modified hyaluronan in physiological conditions. In case of using a diamine or compounds or polymers containing three or more amino groups, it is possible to prepare crosslinked hyaluronan derivatives. The described solution brings along a significant advantage not only in the field of carriers of biologically active substances, but also in tissue engineering where crosslinking with biologically acceptable amino compounds in physiological conditions is very much demanded.;

主权项：1. Hyaluronic acid derivative modified by a double bond in the positions 4 and 5 of the glucosamine part of the polysaccharide and at the same time oxidized to an aldehyde in the position 6 of the glucosamine part of the polysaccharide, according to the structural formula X, or a hydrated form thereof according to the structural formula Ywherein R is hydrogen, any metal cation or an organic cation.