GENETIC MARKERS FOR IDENTIFYING VIRULENCE OF STREPTOCOCCUS INIAE AND IDENTIFYING METHOD USING PEPTIDE NUCLEIC ACIDS AND REAL-TIME PCR,申请号KR20140180340-传众专利搜索

GENETIC MARKERS FOR IDENTIFYING VIRULENCE OF STREPTOCOCCUS INIAE AND IDENTIFYING METHOD USING PEPTIDE NUCLEIC ACIDS AND REAL-TIME PCR

申请公布号：KR101531296(B1)

申请号：KR20140180340

申请日期：2014.12.15

申请公布日期：2015.06.24

申请人：

REPUBLIC OF KOREA(NATIONAL FISHERIES RESEARCH ANDDEVELOPMENT INSTITUTE)

发明人：KIM, MYOUNG SUG;CHOI, HYE SUNG;JUNG, SUNG HEE;HAN, HYUN JA;DO, JEUNG WAN

分类号：C12Q1/68

主分类号：C12Q1/68

摘要：The present invention relates to a probe for identifying a genotype of Streptococcus iniae capable of hybridizing with fragment sequences, which includes a T368C or T385G single nucleotide polymorphism mutation in a cpsD sequence of SEQ ID NO: 1 Streptococcus iniae under stringent conditions, and more specifically, relates to a kit for identifying the virulence of Streptococcus inae simply, quickly, and precisely by allowing genotypes affecting the virulence to exhibit a different melting temperature for each genotype using peptide nucleic acids (PNA) with excellent coherence with DNA.