SYNTHETIC OLIGONUCLEOTIDE SET FOR DETECTING DNA OF PERIODONTAL-PATHOGENIC MICROORGANISM Tannerella forsythensis BY POLYMERASE CHAIN REACTION,申请号RU20120106528-传众专利搜索

SYNTHETIC OLIGONUCLEOTIDE SET FOR DETECTING DNA OF PERIODONTAL-PATHOGENIC MICROORGANISM Tannerella forsythensis BY POLYMERASE CHAIN REACTION

申请公布号：RU2535988(C2)

申请号：RU20120106528

申请日期：2012.02.24

申请公布日期：2014.12.20

申请人：

ZAKRYTOE AKTSIONERNOE OBSHCHESTVO "NAUCHNO-PROIZVODSTVENNAJA FIRMA DNK-TEKHNOLOGIJA"

发明人：REBRIKOV DENIS VLADIMIROVICH;ZORINA OKSANA ALEKSANDROVNA;PETRUKHINA NATALIJA BORISOVNA;BORISKINA OL'GA ANDREEVNA;BERKUTOVA IRINA SERGEEVNA;AJMADINOVA NELLI KAMIL'EVNA

分类号：C12Q1/68;C12N15/11

主分类号：C12Q1/68

摘要：FIELD: chemistry.SUBSTANCE: invention relates to biochemistry, particularly a synthetic oligonucleotide set for detecting DNA of periodontal-pathogenic microorganism Tannerella forsythensis via a polymerase chain reaction. Said set includes Tannerella forsythensis gene bspA fragment specific primers 5'-TGGCACCCTCCGATGCCGAC-3' and 5'-GCGCAGACCGTTGGGTTTCA-3', as well as a probe (BHQ1)-5'-CCGCGCCCGA(FdT)GCGTCGCTGGC-3'-P, wheree BHQ1 denotes a dark fluorescence extinguisher attached to the 5'-terminal nucleotide, and FdT is a fluorescent dye FAM attached to the nucleotide T.EFFECT: present invention provides reliable detection of presence of Tannerella forsythensis in biological material.