MODULAR BASE-SPECIFIC NUCLEIC ACID BINDING DOMAINS FROM BURKHOLDERIA RHIZOXINICA PROTEINS,申请号US201313949880-传众专利搜索

MODULAR BASE-SPECIFIC NUCLEIC ACID BINDING DOMAINS FROM BURKHOLDERIA RHIZOXINICA PROTEINS

申请公布号：US2014230083(A1)

申请号：US201313949880

申请日期：2013.07.24

申请公布日期：2014.08.14

申请人：

CELLECTIS

发明人：Bertonati Claudia;Duchateau Philippe;Juillerat Alexandre;Silva George;Valton Julien

分类号：C12N15/87;A01K67/027

主分类号：C12N15/87

地址：Paris FR

摘要：The present invention concerns new modular base-per-base specific nucleic acid binding domains (MBBBD) derived from newly identified proteins from the bacterial endosymbiont Burkholderia Rhizoxinica and their use for engineering nucleic acid processing enzymes, such as specific endonucleases or transcription activators.

主权项：1. A nucleic acid binding polypeptide that comprises a succession of modules from 30 to 35 amino acids, said succession of modules displaying a base per base specificity towards a nucleic acid target sequence, wherein at least one of said modules has at least 70% sequence identity with one of the module polypeptide sequences from the proteins E5AV36, E5AW43 and E5AW45 of Burkholderia rhizoxinica.