METHOD FOR DETERMINING INTRINSIC BINDING PARAMETERS OF AN ANALYTE TO A LIGAND, A METHOD FOR SELECTING AN ANALYTE FROM A GROUP OF ANALYTS, THE SELECTED LIGAND OR ANALYTE, AND SENSOR,申请号EP20120719773-传众专利搜索

METHOD FOR DETERMINING INTRINSIC BINDING PARAMETERS OF AN ANALYTE TO A LIGAND, A METHOD FOR SELECTING AN ANALYTE FROM A GROUP OF ANALYTS, THE SELECTED LIGAND OR ANALYTE, AND SENSOR

申请公布号：EP2707715(A1)

申请号：EP20120719773

申请日期：2012.05.11

申请公布日期：2014.03.19

申请人：

SSENS B.V.;IBIS TECHNOLOGIES BV

发明人：SCHASFOORT, RICHARD BERNARDUS MARIA;ENGBERS, GERARD HENRICUS MARIA

分类号：G01N33/543;G01N33/557

主分类号：G01N33/543

摘要：<p>The present invention relates to a method for determining intrinsic binding parameters, such as KD, kd and ka, of an analyte to a ligand, such as a drug and a protein, a drug and a receptor, and an antibody and antigen, wherein the maximal binding response Rmax or RL and at least one binding parameter is determined at at least two different ligand surface densities present on a sensor support, and extrapolating the value of the binding parameter to ligand density=0, characterized by Rmax=0 or R1=0, to a method of selecting an analyte and/or ligand, and to the selected ligand, analyte and a sensor.</p>