METHOD FOR MEASURING CHOLESTEROL IN HDL SUBFRACTION, AND REAGENTS AND KIT THEREFOR,申请号US201214002816-传众专利搜索

METHOD FOR MEASURING CHOLESTEROL IN HDL SUBFRACTION, AND REAGENTS AND KIT THEREFOR

申请公布号：US2013344518(A1)

申请号：US201214002816

申请日期：2012.03.15

申请公布日期：2013.12.26

申请人：

KATAYAMA YUKI;SUGIUCHI HIROYUKI;MATSUSHIMA KAZUMI;KUMAMOTO HEALTH SCIENCE UNIVERSITY;KYOWA MEDEX CO., LTD.

发明人：KATAYAMA YUKI;SUGIUCHI HIROYUKI;MATSUSHIMA KAZUMI

分类号：C12Q1/60

主分类号：C12Q1/60

摘要：The present invention provides a method for simply and precisely measuring cholesterol in an HDL subfraction contained in a sample. This is a method for measuring cholesterol in HDL3 contained in a sample, which comprises reacting a sample with (1) a combination of a cholesterol ester hydrolase and a cholesterol oxidase or (2) a combination of a cholesterol ester hydrolase, an oxidized coenzyme and a cholesterol dehydrogenase in an aqueous medium containing: (a) a divalent metal salt; (b) an alkali metal salt selected from the group consisting of a sulfate, a nitrate, a carbonate, an acetate and a halide; and (c) dextran sulfate or a salt thereof, and measuring a substance formed or consumed in the reaction without separating and removing lipoproteins other than HDL3.