HIGH THROUGHPUT SCREENING OF MUTAGENIZED POPULATIONS,申请号EP20060783930-传众专利搜索

HIGH THROUGHPUT SCREENING OF MUTAGENIZED POPULATIONS

申请公布号：EP1929039(B2)

申请号：EP20060783930

申请日期：2006.09.21

申请公布日期：2013.11.20

申请人：

KEYGENE N.V.

发明人：VAN EIJK, MICHAEL, JOSEPHUS, THERESIA;VAN TUNEN, ADRIANUS, JOHANNES

分类号：C12Q1/68

主分类号：C12Q1/68

摘要：Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.