SEQUENCE-SPECIFIC ANALYSIS OF NUCLEIC ACIDS

申请公布号：WO2013045417(A1)

申请号：WO2012EP68818

申请日期：2012.09.25

申请公布日期：2013.04.04

申请人：

UNIVERSITAET ROSTOCK;FLECHSIG, GERD-UWE;MIX, MAREN;KRUEGER, SIMONE

发明人：FLECHSIG, GERD-UWE;MIX, MAREN;KRUEGER, SIMONE

分类号：C12Q1/68

主分类号：C12Q1/68

摘要：The invention relates to a method for the sequence-specific analysis of nucleic acids in a sample in which the nucleic acid is present at least in part as a double strand, which method uses electrochemical detection methods (e.g. with [OsO4(bipy)]). In particular, the methods comprise steps in which the at least partially double-stranded nucleic acid strands are converted by thermal denaturation to single strands which are termed target strands, and at least one nucleic acid strand designated protective strand is added, which protective strand can hybridise with a target strand, in order to form partial double-stranded sections, wherein the protective strands are shorter than the target strands, wherein the temperature of the sample is rapidly lowered to a temperature of below 5°C, preferably below 0°C. The invention also relates to devices which are suitable for these methods comprising a flow system in which the steps of the method can take place one after the other, having heatable sections for thermal denaturation and also coolable sections for rapid cooling of the sample.

AZETIDINONES

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