Method for introducing a sample specific DNA tag into a plurality of DNA fragments from a plurality of samples,申请号US20100728071-传众专利搜索

Method for introducing a sample specific DNA tag into a plurality of DNA fragments from a plurality of samples

申请公布号：US8318434(B2)

申请号：US20100728071

申请日期：2010.03.19

申请公布日期：2012.11.27

申请人：

CUPPENS HARRY;KATHOLIEKE UNIVERSITEIT LEUVEN, K.U.LEUVEN R&D

发明人：CUPPENS HARRY

分类号：C12Q1/68;C12P19/34

主分类号：C12Q1/68

摘要：The invention discloses methods for introducing a sample specific DNA tag into a plurality of DNA fragments from a plurality of samples. For each of the samples, the method involves (1) amplifying DNA fragments in a first multiplex or multiplex-like PCR reaction using amplicon specific forward and reverse primers. Each forward amplicon specific primer including a first adaptor sequence and each reverse amplicon specific primer including a second adaptor. The first adaptor sequence is identical in all forward primers for each of the samples. The second adaptor sequence is identical in all reverse primers for each of the samples, and (2) further amplifying the amplified nucleic acids obtained using one set of forward and reverse sample specific primers which are directed against the first and second adaptor sequences. One or both of the sample specific primers includes a DNA sequence which differs for each of the samples.