Gene Expression Technique
申请公布号:US2012231505(A1)
申请号:US201213474313
申请日期:2012.05.17
申请公布日期:2012.09.13
发明人:FINNIS CHRISTOPHER JOHN ARTHUR;SLEEP DARRELL;SHUTTLEWORTH GILLIAN
分类号:C12P21/00;C12N9/04;C12N9/16;C12N9/64;C12N9/72;C12N9/90;C12P21/08
主分类号:C12P21/00
摘要:The present invention provides a method for producing a desired protein (such as a desired heterologous protein) comprising: (a) providing a host cell comprising a first recombinant gene encoding a protein comprising the sequence of a first chaperone protein, a second recombinant gene encoding a protein comprising the sequence of a second chaperone protein and a third gene, such as a third recombinant gene, encoding a desired protein (such as a desired heterologous protein), wherein the first and second chaperones are different; and (b) culturing the host cell in a culture medium to obtain expression of the first, second and third genes.