NOVEL HOT START NUCLEIC ACID AMPLIFICATION,申请号US201113074752-传众专利搜索

NOVEL HOT START NUCLEIC ACID AMPLIFICATION

申请公布号：US2011189736(A1)

申请号：US201113074752

申请日期：2011.03.29

申请公布日期：2011.08.04

申请人：

AFFYMETRIX, INC.

发明人：KUBU CHRISTOPHER J.;MULLER-GREVEN JEANNINE C.;MOFFETT ROBERT B.

分类号：C12P19/34;C12N9/10

主分类号：C12P19/34

摘要：Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.