Rapid detection of replicating cells

申请公布号：EP2311934(A2)

申请号：EP20100013064

申请日期：2002.09.06

申请公布日期：2011.04.20

申请人：

GENOMIC PROFILING SYSTEMS, INC.

发明人：STRAUS, DON

分类号：C12M1/34;C12Q1/00;C12Q1/04;C12Q1/06;C12Q1/18;C12Q1/68;C12Q1/70;G01N1/30;G01N15/14;G01N21/76;G01N21/78;G01N33/48;G01N33/50;G01N33/53;G01N33/543;G01N33/545;G01N33/554;G01N33/567;G01N33/569;G01N33/58

主分类号：C12M1/34

摘要：The invention provides efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology - the long time needed for detection using traditional methods - while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

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