Method of screening candidate compounds for susceptibility to biliary excretion in hepatocyte cell cultures
申请公布号:NZ551420(A)
申请号:NZ20050551420
申请日期:2005.05.10
申请公布日期:2011.02.25
发明人:BROUWER, KIM L;TIAN, XIANBIN;ZHANG, PEIJIN
分类号:C12Q1/00
主分类号:C12Q1/00
摘要:A method of screening a candidate compound for susceptibility to biliary excretion by a hepatocyte transport protein, the method comprising: (a) providing a cell culture comprising a plurality of hepatocytes and at least one bile canaliculus, at least one of the hepatocytes comprising a transport protein; (b) exposing a candidate compound to the culture; and (c) determining an amount of the candidate compound in the at least one bile canaliculus to thereby screen the candidate compound for susceptibility to biliary excretion by the hepatocyte transport protein, comprising inhibiting expression of the hepatocyte transport protein and detecting an amount of the candidate compound present in the at least one bile canaliculus, wherein the presence of a reduced amount of the candidate compound in the at least one bile canaliculus as compared to an amount of the candidate compound in the at least one bile canaliculus when expression of the hepatocyte transport protein is not inhibited indicates the susceptibility of the candidate compound to biliary excretion by the hepatocyte transport protein.
ORGANIC ELECTROLUMINESCENCE DEVICE
LIGHT-EMITTING ELEMENT, LIGHT-EMITTING DEVICE AND ELECTRONIC EQUIPMENT
LIGHT-EMITTING MATERIAL, LIGHT-EMITTING ELEMENT, LIGHT-EMITTING DEVICE, AND ELECTRONIC APPARATUS
LIGHT-EMITTING ELEMENT, LIGHT-EMITTING DEVICE, AND ELECTRONIC APPARATUS
BATTERY MONITORING DEVICE AND BATTERY MONITORING METHOD
INSPECTION DEVICE AND INSPECTION METHOD
METHOD OF CONTROLLING HUMIDITY IN MANUFACTURING FACILITY OF ORGANIC EL PANEL
METHOD AND DEVICE FOR GUIDING TERMINAL USING CLOGGING PLUG
CONDUCTIVE POWDER AND FORMING METHOD OF CONDUCTIVE POWDER
OPERATION UNIT STRUCTURE OF CIRCUIT BREAKER
IONIZER WITH CORONA DISCHARGE MONITORING FUNCTION
MAGNETIC STRUCTURE, MAGNETIC SHIELD, AND MAGNETIC POLE USED FOR MAGNETIC RECORDING HEAD
MAGNETIC TAPE AND MAGNETIC TAPE CARTRIDGE
POSITION TRACKING SYSTEM, GAME CONTROL METHOD, AND SERVICE PROVIDING SYSTEM
STRUCTURED DATA STORAGE DEVICE, STRUCTURED DATA STORAGE PROGRAM, AND STRUCTURED DATA STORAGE METHOD
CAPACITOR MISMOUNTING DETECTOR AND CAPACITOR MISMOUNTING DETECTING METHOD
UPDATE INFORMATION DISPLAY PROGRAM AND UPDATE INFORMATION DISPLAY METHOD