METHOD FOR EVALUATING EXPRESSION DEGREE OF ANTI-FUNGAL ACTION BY TEST SUBSTANCE THAT MIGRATED INTO TISSUE
申请公布号:JP2008061519(A)
申请号:JP20060240144
申请日期:2006.09.05
申请公布日期:2008.03.21
发明人:KUBOTA NOBUO;MIKI TOYOHIKO;MIYAMAE AKIKO
分类号:C12Q1/18;G01N33/15
主分类号:C12Q1/18
摘要:PROBLEM TO BE SOLVED: To provide a method for screening a drug for treating mycoses, that takes into consideration the biological factors of the host. SOLUTION: The method for evaluating the expression degree of anti-fungal action with a test substance that has migrated into tissues comprises administering the test substance into an animal (excluding human beings), collecting a target organ, cutting out a plurality of slices having order of depth from the target organ, contacting and culturing the cut slices in a solid culture medium inoculated with a fungus, and then evaluating that the test sample exists at the position of the cut tissues in an amount exceeding an effective dose, when the size of an inhibition circle formed on the solid culture medium and used as an index is large, or that the test sample exists at the position of the cut tissue, in an amount smaller than the effective dose, when the blocking circle is small or is not generated. COPYRIGHT: (C)2008,JPO&INPIT
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