METHODS OF USING CLEAVABLE SOLID PHASES FOR ISOLATING NUCLEIC ACIDS,申请号KR20077001037-传众专利搜索

METHODS OF USING CLEAVABLE SOLID PHASES FOR ISOLATING NUCLEIC ACIDS

申请公布号：KR20070058433(A)

申请号：KR20077001037

申请日期：2007.01.15

申请公布日期：2007.06.08

申请人：

LUMIGEN, INC.

发明人：AKHAVAN TAFTI HASHEM;DESILVA RENUKA;CILLI NICOLE M.;CRIPPS WILLIAM G.;HANDLEY RICHARD S.;O'CONNOR ELIZABETH A.;REDDY LEKKALA V.;SIRIPURAPU SARADA

分类号：C12Q1/68;C07H21/00;C07H21/02;C07H21/04

主分类号：C12Q1/68

摘要：<p>Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or micowells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.</p>