DETECCION DE SACAROSA MEDIANTE ANALISIS INMUNOSORBENTE LIGADO A ENZIMA.

申请公布号：ES2221210(T3)

申请号：ES19980951142T

申请日期：1998.11.02

申请公布日期：2004.12.16

申请人：

DE NOVO ENZYME CORPORATION

发明人：BORGFORD, THOR, JON;RACHER, KATHLEEN, IRIS;BRAUN, CURTIS, ARCHIE, JOHN

分类号：G01N33/53;C12Q1/48;C12Q1/54;G01N33/66;(IPC1-7):C12Q1/48

主分类号：G01N33/53

摘要：Un método para analizar sacarosa en un fluido biológico; comprendiendo dicho método las etapas de: (a) revestir un sustrato con (i) un polímero de glucosa o fructosa seleccionado del grupo que consiste en amilosa, dextrano y levano y (ii) una enzima de sacarosa transglucosidasa correspondiente a dicho polímero de glucosa o fructosa seleccionado; (b) incubar el sustrato revestido con un fluido biológico; y (c) determinar una cantidad de sacarosa presente en el fluido midiendo un incremento en una cantidad del polímero de glucosa o fructosa.

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