Method for analysis of substances in tissue or in cells,申请号US20020220345-传众专利搜索

Method for analysis of substances in tissue or in cells

申请公布号：US2004137420(A1)

申请号：US20020220345

申请日期：2002.12.13

申请公布日期：2004.07.15

申请人：

YASUDA AKIKAZU;YASUDA YOSHIMI;NAKAJIMA TERUMI

发明人：YASUDA AKIKAZU;YASUDA YOSHIMI;NAKAJIMA TERUMI

分类号：G01N27/62;B01D59/44;C12N15/09;C12Q1/00;C12Q1/68;G01N33/483;G01N33/68;(IPC1-7):C12Q1/00

主分类号：G01N27/62

摘要：An improved method for directly identifying a chemical structure of a substance present in tissue or cells of organisms is disclosed. In particularly, the method for directly identifying a chemical structure of a substance present in tissue or cells of various kind of organisms comprises the following steps: (1) a step irradiating a laser to a certain intracellular region of a sample tissue slice or a cell, and analyzing the generating mass ions to obtain mass spectrum of the substance present at the cite, (2) a step analyzing the mass spectrum to obtain a mass profile of the substance existing at the intracellular region of the sample tissue slice or in cell, and (3) a step determining the chemical structure of the substance corresponding a certain molecular weight appeared in the mass profile. The method of this invention is conducted by utilizing the combined techniques of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and on-line capillary reversed-phase HPLC/quadrupole orthogonal acceleration time-of-flight (Q-Tof)-MS, and molecular cloning, is disclosed.