Methods and compositions for making emamectin.
申请公布号:ZA200307737(B)
申请号:ZA20030007737
申请日期:2003.10.03
申请公布日期:2004.04.21
发明人:ISTVAN MOLNAR;LIGON, JAMES, MADISON;ZIRKLE, ROSS, ERIC;PHILIP EUGENE HAMMER;HILL, DWIGHT, STEVEN;PACHLATKO, JOHANNES, PAUL;BUCKEL, THOMAS, GUENTER
分类号:C12N15/09;C07K16/40;C12N1/21;C12N9/02;C12N15/53;C12P17/16;C12P17/18;C12P21/00
主分类号:C12N15/09
摘要:Disclosed is a family of P450 monooxygenases, each member of which regioselectively oxidizes avermectin to 4''-keto-avermectin. The P450 monooxgenases find use in methods and formulations for making emamectin from avermectin. Also disclosed are methods for purifying the P450 monooxygenases of the invention, binding agents that specifically bind to the P450 monooxygenases of the invention, and genetically engineered cells that express the P450 monooxygenases of the invention. Also disclosed are ferredoxins and ferredoxin reductases that are active with the P450 monooxygenases of the invention.
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