POLYPEPTIDES WITH A SIGNAL SEQUENCE COMPRISING AN FIVSI MOTIF AND OLYNUCLEOTIDES ENCODING THEREFOR,申请号WO2003GB03273-传众专利搜索

POLYPEPTIDES WITH A SIGNAL SEQUENCE COMPRISING AN FIVSI MOTIF AND OLYNUCLEOTIDES ENCODING THEREFOR

申请公布号：WO2004009819(A3)

申请号：WO2003GB03273

申请日期：2003.07.23

申请公布日期：2004.03.25

申请人：

DELTA BIOTECHNOLOGY LIMITED;SLEEP, DARRELL

发明人：SLEEP, DARRELL

分类号：C07K14/765;C07K14/79;C12N15/62;C12N15/81

主分类号：C07K14/765

摘要：The present invention provides a polypeptide comprising (i) a leader sequence, the leader sequence comprising a (a) secretion pre sequence, and (b) the following motif: -X1-X2-X3-X4-X5- where X1 is phenylalanine, tryptophan, or tyrosine, X2 is isoleucine, leucine, valine, alanine or methionine, X3 is leucine, valine, alanine or methionine, X4 is serine or threonine and X5 is isoleucine, valine, alanine or methionine; and (ii) a desired protein heterologous to the leader sequence. A polypeptide of the invention may additionally comprise, as part of the leader sequence, a secretion pro sequence. The invention also provides a polynucleotide comprising a sequence that encodes a polypeptide of the invention and a cell, preferably a yeast cell, comprising said polynucleotide.