Methods for enhancing the production of viral vaccines in cell culture

申请公布号：US6686190(B2)

申请号：US20000737126

申请日期：2000.12.13

申请公布日期：2004.02.03

申请人：

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人：LAU ALLAN S.

分类号：C12N15/09;A61K38/00;A61K39/12;A61K39/125;A61K39/145;A61K39/205;C07K14/47;C07K14/555;C12N7/00;C12N7/01;C12N9/12;C12N15/113;C12N15/19;C12N15/67;C12P21/02;C12Q1/18;C12Q1/70;C12R1/91;(IPC1-7):C12N7/01;C12N7/02;C12N5/00;C07H21/04;C07H21/02;C07H21/00

主分类号：C12N15/09

摘要：Methods for enhancing the production of viral vaccines in animal cell culture are described. These methods rely on the manipulation of the cellular levels of certain interferon induced antiviral activities, in particular, cellular levels of double-stranded RNA (dsRNA) dependent kinase (PKR) and 2'-5' oligoadenylate synthetase (2-5A synthetase). In cell cultures deficient for PKR or 2-5A synthetase, viral yield is enhanced by several orders of magnitude over cell cultures with normal levels of these proteins making these cell cultures useful for the production of viral vaccines.

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