GENE-TRAPPING CONSTRUCT FOR THE IDENTIFICATION AND ISOLATIONOF GENES
申请公布号:CA2274092(C)
申请号:CA19972274092
申请日期:1997.12.05
申请公布日期:2003.03.25
发明人:VON MELCHNER, HARALD
分类号:C12N15/09;C12N5/10;C12N5/16;C12N15/10;C12N15/24;C12N15/55;C12N15/65;C12N15/85;C12Q1/68;(IPC1-7):C12N15/65
主分类号:C12N15/09
摘要:The invention relates to a gene trap construct containing a first reporter gene, which, after activation, can activate a second reporter gene, and the use of this gene trap construct for identification and/or isolation of genes, in particular transiently expressed genes. The invention relates further to a cell, preferably a mamma l cell, containing the above-mentioned gene trap construct. The invention relates furthermore to the use of this mammal cell for identification and/or isolation of genes, in particular transient genes. In addition, the invention relates to a vector containing t he above- mentioned gene trap construction, as well as a kit for identifying and/or isolating genes, in particular transient genes, which contains at least the above-mentioned gene trap construct or the above-mentioned vector. Finally, the invention relates to a method for the identification and/or isolation of genes, in particular transient genes.
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