Anvendelse av ekspresjonskassett i multigensystemer, ekspresjonskassett, rekombinant vertscelle, fremgangsmåte for fremstilling av enblanding av eksogeneproteiner, fremgangsmåte for selektiv biokjemisk oksydasjon, samtfremgangsmåtefor osydas,申请号NO19900004791-传众专利搜索

Anvendelse av ekspresjonskassett i multigensystemer, ekspresjonskassett, rekombinant vertscelle, fremgangsmåte for fremstilling av enblanding av eksogeneproteiner, fremgangsmåte for selektiv biokjemisk oksydasjon, samtfremgangsmåtefor osydas

申请公布号：NO314267(B1)

申请号：NO19900004791

申请日期：1990.11.05

申请公布日期：2003.02.24

申请人：

ROUSSEL-UCLAF

发明人：SLIJKHUIS, HERMAN;SELTEN, GERARDUS CORNELIS MARIA;SMAAL, ERIC BASTIAAN

分类号：C12N15/00;A61K31/57;A61P5/00;C07J5/00;C07J7/00;C07J13/00;C07J21/00;C07J41/00;C07J75/00;C12N1/19;C12N1/21;C12N9/02;C12N9/04;C12N15/09;C12N15/53;C12P33/00;C12P33/16;C12R1/07;C12R1/645;C12R1/85

主分类号：C12N15/00

摘要：Genetically engineered host cells containing new expression cassettes are provided which are able to carry out biochemical oxidations of steroids. In particular the oxidation is carried out with cells into which DNA has been introduced which encodes protein involved in the biological pathway of cholesterol to hydrocortisone. Suited host cells comprise species of Bacillus, Saccharomyces or Kluyveromyces. The new host cells are suited for microbiological oxidations of cholesterol, pregnenolone, progesterone, 17.alpha.-hydroxy-progesterone, and cortexolone, which are intermediates in said biological pathway. The new expression cassettes are also useful in the ultimate production of a multigenic system for a one-step conversion of cholesterol into hydrocortisone.