PROCESS FOR PRODUCING ENZYMES HAVING SUPEROXIDE DISMUTASE ACTIVITY, NOVEL SUPEROXIDE DISMUTASE ENZYMES AND NOVEL PHARMACEUTICAL COMPOSITIONS COMPRISING ENZYMES HAVING SUPEROXIDE DISMUTASE ACTIVITY,申请号CA19912088574-传众专利搜索

PROCESS FOR PRODUCING ENZYMES HAVING SUPEROXIDE DISMUTASE ACTIVITY, NOVEL SUPEROXIDE DISMUTASE ENZYMES AND NOVEL PHARMACEUTICAL COMPOSITIONS COMPRISING ENZYMES HAVING SUPEROXIDE DISMUTASE ACTIVITY

申请公布号：CA2088574(C)

申请号：CA19912088574

申请日期：1991.08.02

申请公布日期：2002.12.10

发明人：ATKINSON, ANTHONY;BOWN, KEVIN J.;CHAMBERS, STEPHEN P.;BREHM, JOHN K.;MINTON, NIGEL P.

分类号：A61K38/44;A61K38/00;A61P9/00;C12N1/21;C12N9/02;C12N15/09;C12N15/53;C12N15/71;C12R1/07;C12R1/19;(IPC1-7):C12N15/53

主分类号：A61K38/44

摘要：The genes (sod) encoding Bacillus stearothermophi- lus and Bacillus caldotenax Mn-superoxide dismutase have been cloned in Escherichia coli and their entire nucleotide sequences determined. With the exception of the post- translationally cleaved N-terminal methionine residue, the predicted amino acid sequence of the B. stearothermophi- lus enzyme (BSMnSOD) exhibits 100 % similarity to the previously determined amino acid sequence. The B, caldoe- nax enzyme (BCMnSOD) differs by two amino acids from BSMnSOD. Both recombinant MnSOD's were shown to be functionally active in E. coli, both in vitro and in vivo, and were expressed to 47 % of the cells soluble protein by coup- ling their transcription to the E. coli trp promoter. The pharmaceutical use of these bacterial superoxide dismu- tases is described.