NOVEL NUCLEOTID SEQUENCES CODING FOR THE ZWA1 GENE

申请公布号：HU0004880(A2)

申请号：HU20000004880

申请日期：2000.12.08

申请公布日期：2002.10.28

申请人：

DEGUSSA-HUELS AKTIENGESELLSCHAFT

发明人：DR. BATHE BRIGITTE;DR. KALINOWSKI JOERN;DR. MARX ACHIM;DR. MOECKEL BETTINA;DR. PFEFFERLE WALTER;DR. PUEHLER ALFRED

分类号：C12N15/09;C07H21/00;C07K14/34;C12N1/21;C12N9/00;C12N15/11;C12N15/31;C12N15/53;C12P13/08;C12Q1/68;C12R1/15

主分类号：C12N15/09

摘要：Isolated polynucleotide (I) is the new zwa1 gene from Corynebacterium glutamicum. Isolated zwa1 polynucleotide (I) contains a sequence that is: (i) at least 70% identical with a sequence that encodes a 199 amino acid (aa) sequence (II), given in the specification; (ii) encodes a polypeptide at least 70% identical with (II); (iii) is the complement of (i) or (ii); or (iv) contains at least 15 consecutive nucleotides (nt) from (i)-(iii). Independent claims are also included for the following: (1) vectors containing (I); (2) coryneform bacterial host cells containing the vector of (1) or having the zwa1 gene amplified; and (3) production of L-amino acids, especially L-lysine, by growing an aa-producing bacterium in which at least the zwa1 gene has been amplified.