METHOD FOR THE EFFECTIVE INSERTION OF GROUP II INTRONS INTO SPECIFIC TARGET DNA SITES

申请公布号：WO2002059362(A1)

申请号：ES2002000030

申请日期：2002.01.24

申请公布日期：2002.08.01

摘要：<p>The invention relates to a method for inserting a nucleic acid molecule into any specific target DNA sites. The method comprises the following steps: the introduction of the group II intron RNA which contains two nucleotide sequences capable of hybridising with two sequences contained in one of the strands of the DNA substrate; the modification of the intron RNA sequences, which hybridise with the substrate DNA, such that the specific sequences in one of the strands of a given DNA substrate can be identified; and the addition of a protein coded by the intron which, in its wild form, has no endonuclease domain. The reengineered group II intron is suitable for any in vivo or in vitro application and particularly for mutagenesis.</p>

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