Attachment of oligonucleotides to solid supports through schiff base type linkages for capture and detection of nucleic acids,申请号US20010780954-传众专利搜索

Attachment of oligonucleotides to solid supports through schiff base type linkages for capture and detection of nucleic acids

申请公布号：US2002081591(A1)

申请号：US20010780954

申请日期：2001.02.09

申请公布日期：2002.06.27

申请人：

LUKHTANOV EUGENY ALEXANDER;PODYMINOGIN MIKHAIL A.;HEDGPETH JOEL

发明人：LUKHTANOV EUGENY ALEXANDER;PODYMINOGIN MIKHAIL A.;HEDGPETH JOEL

分类号：C12N15/09;C07B61/00;C07F9/24;C07H21/00;(IPC1-7):C12Q1/68;C07H21/04;C12M1/34

主分类号：C12N15/09

摘要：Derivatized oligonucleotides (ODNs) are coupled to a solid support in improved yield resulting in a high density of coupled oligonucleotide per surface unit of the support, through a Schiff base type bond formed between an NH2 group attached either to the solid support or to the ODN and an aromatic aldehyde attached to the other of the solid support and the ODN. The preferred solid support-ODN conjugate is formed between semicarbazide groups attached to a glass surface and an aromatic aldehyde attached at either 3', or 5' end of an ODN or to an intermediate nucleotide of the ODN.