Method for the preparation of an expression vector for the production of therapeutic proteins in transgenic animals
申请公布号:EP1217072(A2)
申请号:EP20010128498
申请日期:2001.12.07
申请公布日期:2002.06.26
发明人:MIKUS, TOMAS;MALY, PETR
分类号:C12N15/66;C07K14/505;C12N15/85;(IPC1-7):C12N15/66;C12N15/67;C12N5/16;C12P21/02;A01K67/00
主分类号:C12N15/66
摘要:<p>The solution refers to the way of vector construction, based on the utilization of modified translational Kozak consensus to connect promoter regulatory sequence with a coding structural gene fragment. The recombinant plasmid vector prWhEBS-BNX was constructed. For connection of the gene genome sequence for hEPO with the promoter of gene for rWAP the original motif of translation Kozak consensus is modified by installation of NotI restriction site. In this way prepared expression vector prWhEBS-BNX is transferred to genome of eukaryotic cells and genome of vertebrates for production of a polypeptide product presenting the entire primary structural conformation of human erythropoietin.</p>
Multiple blood bag having plasticizer-free portions and a high blood component survival rate
Cooling system for internal combustion engine
Conical roller chain indexing device
Magnetic pickup arrangement for stringed musical instrument
Selector pin assembly for manual transmission shifter
Method and apparatus for reducing nitrous oxide emissions from combustors
Method for automatically assembling articles, such as clips
Apparatus for the skinning of fish fillets
Apparatus for curing photo-developing inks
INJECTION FUEL SUPPLYING SYSTEM FOR ROTARY ENGINE
Therapeutic compositions containing polymers having polyunsaturated acid radicals