Method for the preparation of an expression vector for the production of therapeutic proteins in transgenic animals,申请号EP20010128498-传众专利搜索

Method for the preparation of an expression vector for the production of therapeutic proteins in transgenic animals

申请公布号：EP1217072(A2)

申请号：EP20010128498

申请日期：2001.12.07

申请公布日期：2002.06.26

申请人：

BIOPHARM

发明人：MIKUS, TOMAS;MALY, PETR

分类号：C12N15/66;C07K14/505;C12N15/85;(IPC1-7):C12N15/66;C12N15/67;C12N5/16;C12P21/02;A01K67/00

主分类号：C12N15/66

摘要：<p>The solution refers to the way of vector construction, based on the utilization of modified translational Kozak consensus to connect promoter regulatory sequence with a coding structural gene fragment. The recombinant plasmid vector prWhEBS-BNX was constructed. For connection of the gene genome sequence for hEPO with the promoter of gene for rWAP the original motif of translation Kozak consensus is modified by installation of NotI restriction site. In this way prepared expression vector prWhEBS-BNX is transferred to genome of eukaryotic cells and genome of vertebrates for production of a polypeptide product presenting the entire primary structural conformation of human erythropoietin.</p>