HIGH SPECIFICITY HOMOCYSTEINASES,申请号CA20002369842-传众专利搜索

HIGH SPECIFICITY HOMOCYSTEINASES

申请公布号：CA2369842(A1)

申请号：CA20002369842

申请日期：2000.06.28

申请公布日期：2001.01.04

申请人：

ANTICANCER, INC.

发明人：XU, MINGXU;TAN, YUYING;HAN, QINGHONG;TANG, LI

分类号：G01N31/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/88;C12N15/09;C12N15/60;C12Q1/00;C12Q1/02;C12Q1/37;C12Q1/48;G01N31/10;G01N33/483;G01N33/50;G01N33/68;(IPC1-7):C12N15/60

主分类号：G01N31/00

摘要：Homocysteinase enzymes are disclosed which have sufficient specificity for homocysteine, as compared to cysteine that hydrogen sulfide can be used as a measure of homocysteine in a biological fluid even in the presence of substantial amounts of cysteine, exceeding the level of homocysteine. The enzyme of desired specificity can be readily prepared by mutation and screening of naturally occuring homocysteinases or by constructing chimeric forms. Also disclosed is a method to identify homocysteinases of the desired specificity with respect to homocysteine and cysteine, as well as an improve d method to assay for hydrogen sulfide by employing a fluorometric readout of a chromophore generated from said hydrogen sulfide. Also included in the scope of the invention is a method to assess the level of cysteine and homocystein e in the same sample.