Storage system comprising an emptied zona pellucida and spermatozoa placed therein and a method of cryopreservation,申请号US19980088392-传众专利搜索

Storage system comprising an emptied zona pellucida and spermatozoa placed therein and a method of cryopreservation

申请公布号：US6132952(A)

申请号：US19980088392

申请日期：1998.06.01

申请公布日期：2000.10.17

申请人：

SAINT BARNABAS MEDICAL CENTER

发明人：COHEN, JACQUES;WILLADSEN, STEEN

分类号：A01N1/02;A61B17/43;(IPC1-7):A01N1/02

主分类号：A01N1/02

摘要：A procedure for cryopreservation and efficient post-thaw recovery of a single or few cells, preferably human spermatozoa, their precursor cells or blastomeres, which is achieved by injecting cells into cell-free mammalian zonae pellucidae. The cells may also be of any other type where there are provided a relatively small number of valuable cells which must be retrieved. The zona pellucida may be cryopreserved with the cells contained therein. The method involves a combination of physical micromanipulation procedures and cryoprotectant mediated cryopropreservation. The zonae may be tracked by positioning them into straws between two small air bubbles prior to preservation. There is a high recovery of zonae and sperm within the zonae, and a high fertilization rate of the sperm using ICSI after recovery.